Recombinant Proteins

Recombinant Kinase Substrates

Field of Application

All kinase substrates can be used in a broad spectrum of biochemical kinase assay technologies including radiometric activity assays (e.g. FlashPlateTM, filter binding assays), in ATP consumptionĀ or antibody-based assays. Substrates have been validated for the use in radiometric assays as well as with the ADP-GloTM kinase assay technology from Promega Corp.

Our Products

Product Product Number Data Sheet Product Description Used at PQ for FlashPlate Assays with Used at PQ for Filter Binding Assays with
ATF2 0594-0000-2
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human, internal fragment, GST-HIS fusion protein, expressed in E.coli

JNK1, JNK2, JNK3, p38-alpha, p38-beta p38-alpha, p38-beta, p38-delta, p38-gamma, JNK3
ELK1 1211-0000-1
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human, full length, GST-HIS fusion protein, expressed in Sf9 cells

ERK2
ERK2 K54R 0416-0000-5
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human, full length, mutationally inactivated (K54R), N-terminally HIS-tagged, expressed in E.coli

MEK1 wt MEK1 wt
JNK1 K55R/K56R 0524-0000-1
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human, full length, mutationally inactivated (K55R, K56R), HIS fusion protein, expressed in E.coli

MKK7
JUN 0718-0000-1
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human, internal fragment, GST fusion protein, expressed in E.coli

JNK1, JNK2
MEK1 K97M 0785-0000-1
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human, full length, mutationally inactivated (K97M), untagged, expressed in Sf9 cells

B-RAF wt, B-RAF V600E, RAF1 Y340/Y341D B-RAF wt, B-RAF V600E, RAF1 Y340/Y341D
p38-alpha K53A 0769-0000-1
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human, full length, mutationally inactivated (K53A), N-terminally HIS-tagged, expressed in E.coli

MKK6 S207D/T211D, SAK MKK6 S207D/T211D
RB-CTF 0040-0000-6
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human, C-terminal fragment, GST fusion protein, expressed in E.coli

MST1, MST2, MST4, NEK2 CDK1/CycA, CDK1/CycB1, CDK1/CycE, CDK2/CycE, CDK3/CycE, CDK4/CycD1, CDK4/CycD3, CDK5/p25NCK, CDK5/p35NCK, CDK6/CycD1, CDK7/CycH/MAT1, CDK9/CycT, GSK3-beta, MST4, NEK6, NLK, PCTAIRE1, VRK1
RB-S6P 0339-0000-5
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GST fusion protein, expressed in E.coli

CDC42BPB, CLK3, MYLK2, ROCK2
RBER-CDC25tide 0590-0000-1
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GST fusion protein, expressed in E.coli

PLK1, PLK3
RBER-CHKtide 0581-0000-5
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GST fusion protein, expressed in E.coli

all CDKs, AMPK-alpha fl, ARK5, CHK1, COT, ERK1, ERK2, GSK3-beta, HIPK1, HIPK3, IKK-beta, MAPKAPK5, MARK1, MARK3, MELK, MINK1, NIK, p38-delta, p38-gamma, PCTAIRE1, PKC-mu, PLK1, RIPK2, TSK2, TTK, VRK1 ERK1, HIPK3, MAPKAPK5, MELK, RIPK2, TTK
RBER-GSK3(14-27) 0349-0000-5
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GST fusion protein, expressed in E.coli

ACV-R1, AKT1, AKT2, AKT3, DAPK1, IKK-epsilon, PIM1, PIM2, PRK1, S6K, SGK1, SGK3, SNK, TGFB-R1
RBER-IRStide 0863-0000-1
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GST fusion protein, expressed in E.coli

CDK8/CycC, p38-gamma CDK8/CycC
RBER-NTRK3tide 1132-0000-1
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GST fusion protein, expressed in E.coli

FGF-R3, TRK-C

Infos in Brief

Item Size

100 Ī¼g, 1 mg, bulk

Global Delivery

Within 2 days after the receipt of a purchase order, shipment free of charge (EU and Switzerland)

Suitability for ATP Consumption Assays

ELK1, JNK1 K55R, K56R, RBER-C9C25 tide are not recommended for use in ATP consumption assays