In Vivo Testing Services

Immuno-Oncology Platform - Proprietary Models

Field of Application

Today, cancer research tends to focus on development of novel cancer immunotherapies. Syngeneic tumor models are advantageous providing a functional immune system to assess novel immunotherapeutic approaches. While classical syngeneic mouse models are based on the implantation of cultured cell lines, ProQinases’ mouse-derived isograft (MDI) tumor models are propagated in mice only. Hence, the major advantage of these novel spontaneous-derived or carcinogen-induced MDI tumor models is the preservation of primary tumor phenotype and intratumoral immune cell populations.

Our Service

Model

Origin

Creation

Efficacy*

RNAseq

Info sheet

JA-0009

adenocarc.

spontaneous

yes

yes

JA-0017

adenocarc.

spontaneous

ongoing

ongoing

JA-2011

sarcoma

carcinogen

yes

yes

JA-2019

sarcoma

carcinogen

yes

ongoing

JA-2041

sarcoma

carcinogen

yes

ongoing

JA-2042

sarcoma

carcinogen

yes

yes

* efficacy study testing immune checkpoint inhibitor(s)

Characteristics of the MDI models

Model

anti-PD1

anti-CTLA-4

Combination

therapeutic window

relevant immune cells

JA-0009

low

low

low

2 weeks

M2 macrophages

JA-2011

low

low

nd

2 weeks

neutrophils

JA-2019

high

high

nd

2 weeks

MDSCs/Tcells

JA-2041

moderate

moderate

nd

3 weeks

MDSCs/Tcells

JA-2042

moderate

moderate

high

2 weeks

Treg cells

JA-0017

nd

nd

nd

>6 weeks

nd

nd = not determined

Infos in Brief

Flow Cytometric Analysis

Analysis of tumor infiltrating leukocytes and cells isolated from spleen and lymph nodes

Three staining panels established: T cells, MDSCs, Macrophages

Customized staining procedures are possible

Standard Study

Comprises among other things:

  1. Tumor implantation;
  2. Measurement of animal weight (3x / week);
  3. Determination of tumor size by calipering;

Optional Services

  1. Characterization of abundance and relative distribution of different immune cell subsets in the tumor and lymphatic tissues by flow cytometry
  2. Blood sampling
  3. Paraffin embedding of tumor tissue
  4. Histological & pathological analysis
  5. Cytokine determination
  6. Provision of tumor tissue for target validation

Related Publications from ProQinase

Poster 1 from AACR meeting 2018, Chicago

Poster 2 from AACR meeting 2018, Chicago