Cellular Assay Services

Cellular Angiogenesis Assay

Field of Application

Angiogenesis, the formation of new blood vessels from pre-existing ones, is a physiological process during growth and development. Beyond that angiogenesis is mandatory for tumor growth and is involved in other pathological disorders (e.g. psoriasis, macular degeneration). The cellular angiogenesis assay mimics the in vivo situation with respect to the first stages of the angiogenic cascade. The assay allows the evaluation of potentially pro- or anti-angiogenic compounds or biologicals.

Infos in Brief

Cells

Primary human umbilical vein endothelial cells (HUVEC), for comparison fibroblasts or smooth muscle cells may be used

Assay Principle

Human EC spheroids are mixed in a collagen or fibrin matrix and transferred into 24 well plates. After polymerization the test compounds and stimulation factors are added

Stimulation

VEGF-A, FGF-2, HB-EGF, Deferoxamine (chemical hypoxia), S1P; other stimulations can be tested upon request

Cpd Exposure

24 h

Readout

Quantification of sprout length (outgrowth from the spheroid)

Testing Formats

IC50 (7 semi-log dilutions in singlicate) or single point measurement (in duplicate)

Cpd Submission

Solved or as pre-weighed powder

Tolerable Solvent

Max 1% DMSO

Reference Cpd

Sunitinib

Report

Comprehensive evaluation of data including raw data for own analysis

Summary Sheet

PDF