Immuno-Oncology Platform - Proprietary Models
Field of Application
Today, cancer research tends to focus on development of novel cancer immunotherapies. Syngeneic tumor models are advantageous providing a functional immune system to assess novel immunotherapeutic approaches. While classical syngeneic mouse models are based on the implantation of cultured cell lines, ProQinases’ mouse-derived isograft (MDI) tumor models are propagated in mice only. Hence, the major advantage of these novel spontaneous-derived or carcinogen-induced MDI tumor models is the preservation of primary tumor phenotype and intratumoral immune cell populations.
Our Service
* efficacy study testing immune checkpoint inhibitor(s)
Characteristics of the MDI models
Model | anti-PD1 | anti-CTLA-4 | Combination | therapeutic window | relevant immune cells |
|---|---|---|---|---|---|
JA-0009 | low | low | low | 2 weeks | M2 macrophages |
JA-2011 | low | low | nd | 2 weeks | neutrophils |
JA-2019 | high | high | nd | 2 weeks | MDSCs/Tcells |
JA-2041 | moderate | moderate | nd | 3 weeks | MDSCs/Tcells |
JA-2042 | moderate | moderate | high | 2 weeks | Treg cells |
JA-0017 | nd | nd | nd | >6 weeks | nd |
nd = not determined
Info in Brief
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Flow Cytometric Analysis
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Analysis of tumor infiltrating leukocytes and cells isolated from spleen and lymph nodes
Three staining panels established: T cells, MDSCs, Macrophages
Customized staining procedures are possible
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Standard Study
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Comprises among other things:
1. Tumor implantation;
2. Measurement of animal weight (3x / week);
3. Determination of tumor size by calipering; -
Sample Report
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Will be provided upon request
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Optional Services
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1. Characterization of abundance and relative distribution of different immune cell
subsets in the tumor and lymphatic tissues by flow cytometry
2. Blood sampling
3. Paraffin embedding of tumor tissue
4. Histological & pathological analysis
5. Cytokine determination
6. Provision of tumor tissue for target validation -
Poster AACR Meeting 2018, Chicago
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New spontaneous and carcinogen-induced mouse-derived isograft (MDI) ...
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Poster AACR Meeting 2018, Chicago
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Value of new spontaneous and carcinogen-induced mouse-derived ...
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Summary Sheet