ProQinase’s cell-based service portfolio comprises target-specific cellular phosphorylation assays as well as phenotypic/functional assays like proliferation assays, soft agar assays (colony formation), 3D tumor spheroid assays, migration assays and a unique cell-based angiogenesis assay. Custom-tailored cellular assays may be established upon request.
Cellular Assay Services
Cellular activity of test compounds can differ from biochemically derived results. Therefore cellular testing should at least be part of rationale drug development. Our cellular phosphorylation assays have been designed to address compound activity in a physiological environment on a physiological substrate.
Cellular proliferation assays are widely used and accepted in oncology as an initial screen in the transition phase from biochemical screens to biological relevant models. ProQinase's CL100-ProliFiler is a standard service package comprising IC50 determination of one test compound against a defined panel of 100 cell lines. In addition, ProQinase offers free choice cell proliferation assay services with definition of the number of test cpds and cell lines according to your requirements.
Anchorage independent growth of cells in soft agar is one of the hallmark characteristics of cellular transformation and uncontrolled cell growth, with normal cells typically not capable of growth in semisolid matrices. Testing drugs in a 3-dimensional (3D) format, like soft agar, is considered superior to using monolayer cultures because cell growth in 3D is more similar to the in vivo cellular environment.
Cell migration is a hallmark of tumor formation and metastatic spreading. The process of cell movement is induced by various agents such as growth factors and chemokines and is associated with complex signaling which involves many components of the cellular motility machinery. The cell migration assay is ideally suited to test the anti-migratory efficacy of test compounds.
This service can be used to analyze the effect of test compounds on three-dimensional cell clones either with mono-spheroids of only tumor cells or with co-spheroids of tumor and stroma cells. Since tumor and stroma cells are labelled with different luciferases the effect of test compounds on tumor and stroma cells can be analyzed separately.
Angiogenesis, the formation of new blood vessels from pre-existing ones, is a physiological process during growth and development. Beyond that angiogenesis is mandatory for tumor growth and is involved in other pathological disorders (e.g. psoriasis, macular degeneration). The cellular angiogenesis assay mimics the in vivo situation with respect to the first stages of the angiogenic cascade. The assay allows the evaluation of potentially pro- or anti-angiogenic compounds or biologicals.
Custom-tailored cellular assay services can be used to address specific questions of customers by using ProQinase’s comprehensive experience in the field of cancer related cell-based assays.