In Vivo Testing Services

In Vivo Hollow Fiber Model

Field of Application

The in vivo hollow fiber model is a fast and economic approach to select a suitable cell line for an in vivo efficacy study. The method allows simultaneous evaluation of test items with three different tumor cell lines under two different conditions (s.c.  &  i.p. implantation) within the same mouse.

Our Service

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Test Mode

Short Description of a Standard Study

1

MTT Readout

Cell culturing; encapsulation of three different cell types in hollow fibers; subcutaneous & intraperitoneal implantation of hollow fibers; in vivo study with treatment (study duration up to 42 days); hollow fiber harvesting; measurement of tumor cell mass by means of MTT assay; protocol & report

2

Luciferase Readout

Cell culturing; encapsulation of three different cell types in hollow fibers; subcutaneous & intraperitoneal implantation of hollow fibers; in vivo study with treatment (study duration up to 42 days); hollow fiber harvesting; measurement of tumor cell mass by means of luciferase activity assay; protocol & report

Infos in Brief

The Test Model

The key element of the assay are semi-permeable fibers which allow access of test items (small molecules, antibodies etc.) to the encapsulated tumor cells.

First Description in the Literature

Hollingshead et al (1995), Life Sciences 57, pp. 131 - 141

Available Cell Lines for MTT Readout

For the MTT variant of the in vivo hollow fiber assay cell lines can be selected from ProQinase’s panels of established subcutaneous and orthotopic tumor models

Available Cell Lines for Luciferase Readout

A subset of ProQinase’s established cell lines for in vivo studies is available as luciferase-transduced variant ready-to-use for hollow fiber assays; please ask for a list

Customer Cell Lines

Studies can also be performed with cell lines provided by our customers

Summary Sheet

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