In Vivo Testing Services

Immuno-Oncology Platform - Standard Models

Field of Application

Our panel of syngeneic models can be used for analysis of immune modulatory drugs as well as for investigation of additional effects of conventional anti-cancer drugs on the immune system. The immuno-oncology platform comprises a panel of syngeneic models and established protocols for the analysis of various cell subtypes of the immune system by flow cytometry. In addition to its standard syngeneic models, ProQinase offers proprietary syngeneic test systems.

Our Service

The following tumor models with immune competent mice are available:

#

Tumor Cell Line

Tissue Origin

Subcutaneous

Intra-mammary

Orthotopic

Metastasis

1

4T1

Breast

Please inquire!

*

4

CT26 wt

Colon

-

5

MC38-CEA#

Colon

-

-

6

RENCA

Kidney

-

8

LL/2

Lung

-

-

11

B16-F10

Skin

**

12

Clone M3

Skin

-

-

9

AB12

Mesothelium

Please inquire!

-

-

2

EMT6

Breast

Please inquire!

Please inquire!

-

7

Hepa1-6

Liver

Please inquire!

-

-

10

Panc02

Pancreas

-

-

Please inquire!

-

3

TuBo

Breast

-

-

Please inquire!

-

* orthotopic implantation, ** intravenous injection
# Carcinoembryonic antigen suited for investigation of tumor-specific T cell response

Infos in Brief

Customer Cell Lines

Additional syngeneic tumor models can be established with cell lines provided by our customers

Flow Cytometric Analysis

- Analysis of tumor infiltrating leukocytes and cells isolated from spleen and lymph
  nodes
- Three staining panels established: T cells, MDSCs, Macrophages
- Customized staining procedures are possible

Tested Checkpoint Inhibitors

Anti-PD-L1, anti-PD-1 and anti-CTLA4 antibodies were evaluated in all subcutaneous models

Standard Study

Comprises among other things:

1. Cell culturing & cell implantation;
2. Measurement of animal weight (3x / week);
3. Determination of tumor size (calipering for s.c., in vivo bioluminescence imaging
    for orthotopic and metastasis models)

Optional Services

1. Characterization of abundance and relative distribution of different immune cell
    subsets in the tumor and lymphatic tissues by flow cytometry
2. Blood sampling
3. Paraffin embedding of tumor tissue
4. Histological & pathological analysis
5. Cytokine determination
6. Provision of tumor tissue for target validation

Summary Sheet

PDF

White Paper

Syngeneic mouse models as a tool to study immune modulatory effects ...

Poster EORTC-NCI-AACR Symposium 2018, Dublin

Prevention of tumor ulceration by tumor cell inoculation into the mammary ...

Poster AACR Meeting 2016, New Orleans

Checkpoint Inhibitor treatment leads to different responses in syngeneic ...