Immuno-Oncology Platform - Standard Models
Field of Application
Our immune-oncology platform can be used for analysis of immune modulatory drugs as well as for investigation of additional effects of conventional drugs on the immune system. The platform comprises a panel of syngeneic tumor models and established protocols for the analysis of various cell subtypes of the immune system by flow cytometry.
Our Service
Infos in Brief
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Customer Cell Lines
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Additional syngeneic tumor models can be established with cell lines provided by our customers
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Flow Cytometric Analysis
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Analysis of tumor infiltrating leukocytes and cells isolated from spleen and lymph nodes
Three staining panels established: T cells, MDSCs, Macrophages
Customized staining procedures are possible
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Tested Checkpoint Inhibitors
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Anti-PD-L1, anti-PD-1 and anti-CTLA4 antibodies were evaluated in all subcutaneous models
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Standard Study
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Comprises among other things:
- Cell culturing & cell implantation;
- Measurement of animal weight (3x / week);
- Determination of tumor size (calipering for s.c., in vivo bioluminescence imaging for orthotopic and metastasis models)
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Optional Services
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- Characterization of abundance and relative distribution of different immune cell subsets in the tumor and lymphatic tissues by flow cytometry
- Blood sampling
- Paraffin embedding of tumor tissue
- Histological & pathological analysis
- Cytokine determination
- Provision of tumor tissue for target validation
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Summary Sheet
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Related Publications from ProQinase
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White Paper (8,5 MB) Poster from AACR meeting 2016, New Orleans