Custom-Tailored Protein Production
Field of Application
If the kinase of your interest is not yet available as off-the-shelf product, we may produce this protein for you according to your specific requirements.
Two Types of Custom-Tailored Protein Production
Type of Production
Description of Work Package
Cloning, expression & purification of 100 μg of your protein of choice Quality control including Coomassie stain and determination of ATP-Km and Vmax (if applicable). In case no substrate is known, we perform a substrate finding approach testing at least 20 generic substrates.
Upscaling of expression and purification of mg amounts of your protein of choice Quality control including Coomassie stain, Mass Specs analysis, Activity measurement (ATP-Km, Vmax, IC50 of reference inhibitor; if available).
Info in Brief
Sf9 insect cells
Additional tags are available
Activation by autophosphorylation
Activation by upstream kinase
Removal of tag
Co-expression with co-factors
Activity Assay Formats
FlashPlate™-based radiometric assay
Filter binding assay
ADP-Glo™ kinase assay, Promega