Cellular Assay Services

Soft Agar Assays (Clonogenic Assays)

Field of Application

Anchorage independent growth of cells in soft agar is one of the hallmark characteristics of cellular transformation and uncontrolled cell growth, with normal cells typically not capable of growth in semisolid matrices. Testing drugs in a 3-dimensional (3D) format, like soft agar, is considered superior to using monolayer cultures because cell growth in 3D is more similar to the in vivo cellular environment.

Our Service

Select Cell Lines

#

Cell specification

Entity of origin

1

J82

Bladder

2

Karpas 299

Blood / Leukemia

3

HL-60

Blood / Leukemia

4

MOLM-13

Blood / Leukemia

5

P31/FUJ

Blood / Leukemia

6

RPMI 8226

Blood / Leukemia

7

U-937

Blood / Leukemia

8

SJSA-1

Bone

9

SK-ES-1

Bone

10

LN229

Brain

11

SK-N-FI

Brain

12

SK-N-MC

Brain

13

SK-N-SH

Brain

14

U118MG

Brain

15

U87MG

Brain

16

CAL-148

Breast

17

EFM-19

Breast

18

EFM-192A

Breast

19

HCC1569

Breast

20

HCC38

Breast

21

KPL-1

Breast

22

HCC 1569

Breast

23

JIMT-1

Breast

24

MCF-7

Breast

25

MDA MB 231

Breast

26

MDA MB 468

Breast

27

SK.BR-3

Breast

28

T47D

Breast

29

ZR-75-1

Breast

30

C33a

Cervix

31

HeLa

Cervix

32

SiHa

Cervix

33

Colo 205

Colon/Rectum

34

CX-1

Colon/Rectum

35

DLD-1

Colon/Rectum

36

HCT116

Colon/Rectum

37

HCT-15

Colon/Rectum

38

HT-29

Colon/Rectum

39

Hutu 80

Colon/Rectum

40

LOVO

Colon/Rectum

41

LS174T

Colon/Rectum

42

RKO

Colon/Rectum

43

SW480

Colon/Rectum

44

SW620

Colon/Rectum

45

SW948

Colon/Rectum

46

T84

Colon/Rectum

47

HEC-1-A

Endometrium

48

HEC-1-B

Endometrium

49

HT-1080

Fibrosarcom

50

A498

Kidney

51

Caki-2

Kidney

52

HepG2

Liver

53

A427

Lung

54

A549

Lung

55

Calu-6

Lung

56

H1299

Lung

57

H460

Lung

58

HCC827

Lung

59

N417

Lung

60

NCI-H441

Lung

61

BEN

Lung

62

CORL279

Lung

63

DV90

Lung

64

NCI-H1048

Lung

65

NCI-H1299

Lung

66

NCI-H1437

Lung

67

NCI-H1573

Lung

68

NCI-H1581

Lung

69

NCI-H1703

Lung

70

NCI-H2009

Lung

71

NCI-H2106

Lung

72

NCI-H2110

Lung

73

NCI-H69

Lung

74

NCI-H838

Lung

75

NCI-H889

Lung

76

RERF-LC-MS

Lung

77

SCLC-21H

Lung

78

SK-LU-1

Lung

79

MCAS

Ovary

80

NCI-ADR

Ovary

81

RL95-2

Ovary

82

SK-OV3

Ovary

83

COV434

Ovary

84

EFO-27

Ovary

85

OV56

Ovary

86

OVK18

Ovary

87

AsPC-1

Pancreas

88

Mia PaCA 2

Pancreas

89

DU-145

Prostate

90

LnCap

Prostate

91

PC3

Prostate

92

A2058

Skin

93

A375

Skin

94

MDA MB 435

Skin

95

SK-MEL-3

Skin

96

Hs 746T

Stomach

97

MKN-1

Stomach

98

MKN-45

Stomach

99

NCI-N87

Stomach

100

SCH

Stomach

101

SNU-1

Stomach

Infos in Brief

Cpd Exposure

7 - 14 days

Readout

Fluorescence of cell viability indicator Resazurin; optional: image of each well

Testing Formats

IC50 (8 semi-log dilutions in duplicate)

Cpd Submission

Solved or as pre-weighed powder

Tolerable Solvent

Max 0.1 % DMSO

Reference Cpd

Actinomycin D

Report

Comprehensive evaluation of data including raw data for own analysis

Summary Sheet

PDF