Cellular Assay Services

Cellular Phosphorylation Assays

Field of Application

Our cellular kinase assays have been designed to measure compound activity in a physiological environment on a physiological substrate. Cellular kinase assays are therefore a crucial part of rationale drug development.

Our Service

Select Phosphorylation Assays

#

Kinase

Cell Line

Reference Compound

Info Sheet

1

AKT1 (h)

Rat1-myrAKT1 (r)

Staurosporine

2

ALK (h)

Karpas-299 (h)

Crizotinib

3

Aurora-B (h)

HT-29 (h)

Staurosporine

4

AXL (h)

MEF-AXL (m)

Sunitinib

5

BCR-ABL

K562

Dasatinib

6

B-RAF-VE (h)

Rat1-B-RAF-VE (r)

Sorafenib

7

EGF-R (h)

A431 (h)

Lapatinib

8

EGF-R G719S Mutant (h)

Rat1 (r)

Lapatinib

9

EGF-R L858R Mutant (h)

Rat1 (r)

Lapatinib

10

EGF-R L861Q Mutant (h)

Rat1 (r)

Lapatinib

11

EGF-R T790M Mutant (h)

Rat1 (r)

Lapatinib

12

EGF-R T790M/L858R Mutant (h)

Rat1 (r)

Lapatinib

17

EGF-R Δ752-759 Mutant (h)

Rat1 (r)

Lapatinib

16

EGF-R Δ747-749/A750P Mutant (h)

Rat1 (r)

Lapatinib

19

EGF-R control wt (h)

Rat1 (r)

Lapatinib

20

EPHB4 (h)

MEF-EPHB4 (m)

Sorafenib

21

ERBB2 (h)

NIH3T3-ERBB2 (m)

Lapatinib

22

ERBB4 (h)

T47D (h)

Lapatinib

23

FAK (h)

MEF-FAK (m)

Staurosporine

24

FGF-R2

Kato III

Vargatef

25

FLT3-wt (h)

MEF-FLT3-wt (m)

Sunitinib

26

FLT3-DY (h)

MEF-FLT3-DY (m)

Sunitinib

27

FLT3-ITD (h)

MEF-FLT3-ITD (m)

Sunitinib

28

FYN (h)

Rat1 (r)

Dasatinib

29

Haspin (GSG2)

HT-29 (h)

CHR-6494

30

IGF1-R (h)

MEF-IGF1-R (m)

Staurosporine

31

KIT (h)

M07e (h)

Sunitinib

32

MET (h)

MKN45 (h)

PHA665752

33

MET D1288H Mutant (h)

Rat1 (r)

BMS777607

34

MET D1228N Mutant (h)

Rat1 (r)

BMS777607

35

MET F1200l Mutant (h)

Rat1 (r)

BMS777607

36

MET M1250T Mutant (h)

Rat1 (r)

BMS777607

37

MET Y1230H Mutant (h)

Rat1 (r)

BMS777607

38

MET Y1230A Mutant (h)

Rat1 (r)

BMS777607

39

MET Y1230C Mutant (h)

Rat1 (r)

BMS777607

40

MET Y1230D Mutant (h)

Rat1 (r)

BMS777607

41

MET control wt (h)

Rat1 (r)

BMS777607

42

MNK1 (h)

Karpas-299 (h)

CGP-57380

43

PDGFR-beta (m)

NIH3T3 (m)

Sunitinib

44

PIM1

HEK293T (h)

AZD1208

45

PIM2

HEK293T (h)

AZD1208

46

PIM3

HEK293T (h)

AZD1208

47

ROCK (r)

A7r5 (r)

Y-27632

48

RON (h)

T47D (h)

Crizotinib

49

S6K (h)

Karpas-299 (h)

Everolimus

50

SRC (h)

MEF-SRC (m)

Dasatinib

51

TIE2 (h)

CHO-TIE2 (ha)

Sorafenib

52

VEGF-R2 (h)

HUE (h)

Sunitinib

53

VEGF-R3 (h)

MEF-VEGF-R3 (m)

Sunitinib

13

EGF-R Δ746-750/C797S

Rat1 (r)

Lapatinib

14

EGF-R Δ746-750/T790M

Rat1 (r)

Lapatinib

15

EGF-R Δ746-750/T790M/C797S

Rat1 (r)

Lapatinib

18

EGF-R T790M/C797S/L858R

Rat (r)

Lapatinib

origin of kinase and cell line: (h) = human, (m) = mouse, (r) = rat, (ha) = hamster

Infos in Brief

Cpd Exposure

90 min

Readout

ELISA (sandwich or solid phase)

Testing Formats

IC50 (8 semi-log dilutions in duplicate)

Cpd Submission

Solved or as pre-weighed powder

Tolerable Solvent

Max 1 % DMSO

Report

Comprehensive evaluation of data including raw
data for own analysis

Summary Sheet

PDF

List of Cell Lines for this Service

PDF

Guidelines for Cpd Submission

EXCEL-FILE