Cellular Assay Services

Cell Migration Assays

Field of Application

Cell migration is a hallmark of tumor formation and metastatic spreading. The process of cell movement is induced by various agents such as growth factors and chemokines and is associated with complex signaling which involves many components of the cellular motility machinery. The cell migration assay is ideally suited to test the anti-migratory efficacy of test compounds.

Our Service

Select Cell Lines

#

Cell specification

Entity of origin

1

J82

Bladder

2

SJSA-1

Bone

3

LN299

Brain

4

SK-N-SH

Brain

5

U118MG

Brain

6

U87MG

Brain

7

MCF-7

Breast

8

MDA-MB 231

Breast

9

HeLa

Cervix

10

DLD-1

Colon

11

HCT116

Colon

12

RKO

Colon

13

HT-1080

Fibrosarcoma

14

A498

Kidney

15

Caki-2

Kidney

16

A427

Lung

17

A549

Lung

18

Calu-6

Lung

19

H1299

Lung

20

HCC827

Lung

21

MCAS

Ovary

22

SK-OV3

Ovary

23

DU-145

Prostate

24

PC3

Prostate

25

A375

Skin

26

A431

Skin

27

Hs746T

Stomach

28

MKN-1

Stomach

Infos in Brief

Cpd Exposure

24 - 48 h

Readout

Fluorescence of cell viability indicator Calcein and image of each well

Testing Formats

IC50 (8 semi-log dilutions in duplicate) or single point measurement (in triplicate)

Cpd Submission

Solved or as pre-weighed powder

Tolerable Solvent

Max 0.1 % DMSO

Reference Cpd

SKI-606

Report

Comprehensive evaluation of data including raw data for own analysis

Summary Sheet

PDF

List of Cell Lines for this Service

PDF