Biochemical Assay Services

Standardized Kinase Profiling Packages

Field of Application

The standardized profiling service packages can be used for the characterization of your test compounds against different kinase panels. Depending on the package type compounds will either be tested with two data points for each kinase (1 conc in duplicate or 2 conc in singlicate) or at 6 or 10 concentrations with calculation of IC50 values.

Our Service

Broad Kinase Panel Selectivity Profiling

#

Type of Package

No. of Kinases

Data Points per Kinase

Description

Info Sheet

1

WildType IC50-Profiler

335

6 conc in singlicate

wildtype forms of protein kinases

2

WildType 2Point-Profiler

335

1 conc in duplicate or 2 conc in singlicate

wildtype forms of protein kinases

3

Mutant Kinase Profiler (add on)

94

1 conc in duplicate or 2 conc in singlicate

disease relevant mutant kinases

4

Lipid Kinase Profiler (add on)

14

1 conc in duplicate or 2 conc in singlicate

wildtype forms of lipid kinases

Read more...

CDK IC50-Profiler

#

Type of Package

No. of Kinases

Data Points per Kinase

Reference Compound

Info Sheet

1

CDK IC50-Profiler

28

10 conc in singlicate

To be selected

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Mutant IC50-Profiler

#

Type of Package

No. of Kinases

Data Points per Kinase

Reference Compound

Info Sheet

1

ABL1 Mutant IC50-Profiler

9

10 conc in singlicate

Imatinib (Gleevec®)

2

ALK Mutant IC50-Profiler

8

10 conc in singlicate

Crizotinib (Xalkori®)

3

EGF-R Mutant IC50-Profiler

11

10 conc in singlicate

Gefitinib (Iressa®)

4

KIT Mutant IC50-Profiler

10

10 conc in singlicate

Imatinib (Gleevec®)

5

MET Mutant IC50-Profiler

10

10 conc in singlicate

PHA665752

6

RET Mutant IC50-Profiler

10

10 conc in singlicate

BIBF1120 (Vargatef®)

Info in Brief

Slots in 2020 for Broad Panel Profiling

2020 - Testing will start on: January 07 // February 03 // March 02 // March 30 // May 04 // June 02 // June 29 // July 27 // August 31 // September 28 // October 26 // November 30

Assay Technologies

Protein kinases: FlashPlate™-based radiometric assay; lipid kinases: ADP-Glo™ assay technology (Promega)

ATP concentration

App. ATP-Km for each kinase

Cpd Submission

100 x Stock solution in 100 % DMSO or
pre-weighed powder (request guidelines)

Report

Detailed report for each project
Sample report will be provided upon request

Turnaround Time

Routinely within 10 working days

Info Sheets

Method: Radiometric Assay, Method: ADP-Glo™ Assay, List of Kinases: Broad Panel Profiling

Guidelines for Cpd Submission

Project Form (WildType 2Point-Profiler)

Project Form (WildType IC50-Profiler)