Biochemical Assay Services

KinaseFinder

Field of Application

The KinaseFinder is a standardized service package for the identification of protein kinases which can phosphorylate a peptide or protein of interest provided by our customer. Kinase hits, which were identified with the standard package, should subsequently be confirmed in a second experiment. The KinaseFinder is ideal for the elucidation of physiological pathways with involvement of protein kinases.

Our Service

Types of KinaseFinder

#

Type of KinaseFinder

No of Protein Kinases

List of Kinases

1

Tyr protein kinases

94

2

Ser/Thr protein kinases

245

3

Ser/Thr & Tyr protein kinases

339

Infos in Brief

Assay Technologies

Analysis of proteins: radiometric filter binding assay; analysis of peptides: radiometric assay with Streptavidin FlashPlates™ (PerkinElmer)

ATP conc

1 μM for all kinases

Testing Formats

Hit identification: 1 data point for each kinase Hit confirmation: 3 substrate conc in triplicate

Substrate Submission

Proteins: as stock solution; peptides: as biotinylated molecules; please, request guidelines

Report

Detailed report for each project

Turnaround Time

Routinely within 10 working days

Reference from a Customer

Please click here...

Summary Sheet

PDF