Biochemical Assay Services

KinaseFinder

Field of Application

The KinaseFinder is a standardized service package for the identification of protein kinases which can phosphorylate a peptide or protein of interest provided by our customer. Kinase hits, which were identified with the standard package, should subsequently be confirmed in a second experiment. The KinaseFinder is ideal for the elucidation of physiological pathways with involvement of protein kinases.

Our Service

Types of KinaseFinder

#

Type of KinaseFinder

No of Protein Kinases

List of Kinases

1

Tyr protein kinases

94

2

Ser/Thr protein kinases

245

3

Ser/Thr & Tyr protein kinases

339

Read more...

Infos in Brief

Assay Technologies

Analysis of proteins: radiometric filter binding assay; analysis of peptides: radiometric assay with Streptavidin FlashPlates™ (PerkinElmer)

ATP conc

1 μM for all kinases

Testing Formats

Hit identification (one protein or peptide concentration in singlicate for each kinase) followed by
Hit confirmation (three protein or peptide concentrations in triplicate for each kinase hit)

Substrate Submission

Proteins: as stock solution; peptides: as biotinylated molecules; please, request guidelines

Report

Detailed report for each project

Turnaround Time

Routinely within 10 working days

Reference from a Customer

Please click here...

Summary Sheet

PDF

Guidelines for Cpd Submission

Guidelines       Project Form      Hit Confirmation Form