Biochemical Assay Services

Kinase SubstrateFinder

Field of Application

The Kinase SubstrateFinder comprises 5 variants of standardized service packages for the identification of proteins or peptides which can serve as a substrate in biochemical activity assays for a certain protein kinase provided by the customer. The 3 generic Kinase SubstrateFinder variants comprise different panels of proteins used as kinase substrate at ProQinase, whereas the physiological SubstrateFinder variants include biotinylated peptide libraries from JPT Peptide Technologies, Berlin, Germany.

Our Service

Types of Kinase SubstrateFinder

#

Type of SubstrateFinder

Protein Substrate

Applicable for

Assay System

1

Y-Generic

19

Tyrosine kinases

ADP-Glo™ kinase assay

2

S/T-Generic

39

Serine/Threonine kinases

ADP-Glo™ kinase assay

3

S/T/Y-Generic

58

All protein kinases

ADP-Glo™ kinase assay

4

Y-Physiological

145

All protein kinases

Radiometric 33PanQinase® assay

5

S/T/YPhysiological

720

All protein kinases

Radiometric 33PanQinase® assay

Infos in Brief

Assay Technologies

Generic SubstrateFinder: ADP-GloTM assay (Promega) Physiological SubstrateFinder: radiometric assay with Streptavidin FlashPlates™ (PerkinElmer)   

ATP conc

1 μM for all assays

Testing Formats

Generic SubstrateFinder: 3 substrate conc in singlicate; Physiological SubstrateFinder: 1 peptide conc in singlicate

Kinase Submission

Shipped on dry ice as solution; please request guidelines

Hit Verification Service

Available as customized project

Report

Detailed report for each project

Turnaround Time

Routinely within 10 working days

Summary Sheet

PDF