Biochemical Assay Services

Kinase IC50-Profiler

Field of Application

These standardized service packages can be used for the determination of IC50 values of your test compounds against selected panels of protein kinases with side by side determination of the IC50 value for a reference compound. Standard packages are available for a panel of 28 CDK/Cyclin complexes and 6 oncologically relevant tyrosine kinases plus related mutants.

Our Service

CDK IC50-Profiler

#

Type of IC50-Profiler

No. of Kinases

Reference Compound

Info Sheet

1

CDK IC50-Profiler

28

To be selected

Mutant IC50-Profiler

#

Type of IC50-Profiler

No. of Kinases

Reference Compound

Info Sheet

1

ABL1 Mutant IC50-Profiler

9

Imatinib (Gleevec®)

2

ALK Mutant IC50-Profiler

8

Crizotinib (Xalkori®)

3

EGF-R Mutant IC50-Profiler

11

Gefitinib (Iressa®)

4

KIT Mutant IC50-Profiler

10

Imatinib (Gleevec®)

5

MET Mutant IC50-Profiler

10

PHA665752

6

RET Mutant IC50-Profiler

10

BIBF1120 (Vargatef®)

Infos in Brief

No of Test Cpds in the Standard Package

CDK-Profiler: 4 cpds; Mutant-Profiler: 1 cpd

Assay Technology

FlashPlate™-based radiometric assay

ATP conc

App. ATP-Km for each kinase

Cpd Concentration Range

10 Concentrations in semi-log steps, in singlicate

Cpd Submission

100 x Stock solution in 100 % DMSO or
pre-weighed powder (request guidelines)

Report

Detailed report for each project

Turnaround Time

Routinely within 10 working days