Biochemical Assay Services

Biochemical Assay Services

ProQinase offers different services for compound testing with biochemical kinase tests. Please choose between flexible Free Choice Kinase Assay Projects and several standardized service packages like the Kinase Wildtype-Profiler, Kinase 410-Profiler, the Kinase MutantProfiler, the KinaseFinder or the Kinase SubstrateFinder.

 

Free choice kinase assay projects allow characterization of your test com-pounds in a self-selected panel of biochemical kinase activity assays. For each project you can freely define (i) the number of test compounds, (ii) the kinase panel, (iii) and the number of data points for each test compound (i.e. number of concentrations and replicates).

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The Kinase Wildtype-Profiler and the Kinase 410-Profiler are standardized service packages which can be used for the characterization of your test compounds in broad panels of biochemical kinase activity assays with two data points for each enzyme.

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These standardized service packages can be used for the side by side determination of IC50 values of your test compounds against the wild type form and a group of mutants of a certain protein kinase. The Kinase MutantProfiler service packages are available for 6 oncologically relevant tyrosine kinases. Determination of the IC50 value of a reference compound (see table below) is included.

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The KinaseFinder is a standardized service package for the identification of protein kinases which can phosphorylate a peptide or protein of interest provided by our customer. Kinase hits, which were identified with the standard package, should subsequently be confirmed in a second experiment. The KinaseFinder is ideal for the elucidation of physiological pathways with involvement of protein kinases.

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The Kinase SubstrateFinder comprises 5 variants of standardized service packages for the identification of proteins or peptides which can serve as a substrate in biochemical activity assays for a certain protein kinase provided by the customer. The 3 generic Kinase SubstrateFinder variants comprise different panels of proteins used as kinase substrate at ProQinase, whereas the physiological SubstrateFinder variants include biotinylated peptide libraries from JPT Peptide Technologies, Berlin, Germany.

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The HitDetector service can be used to identify chemical structures which can serve as a starting point for a drug discovery program. The service package comprises biochemical testing of a drug-like small molecule library at one concentration in singlicate against a protein kinase of your choice selected from ProQinase’s protein kinase panel.

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Custom-tailored biochemical assay services can be used to address specific questions of customers by using ProQinase’s comprehensive experience in the field of biochemical kinase assays.

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