In Vivo Testing Services

In Vivo Angiogenesis Model

Field of Application

The spheroid-based in vivo angiogenesis model allows to study the effect of pro- and anti-angiogenic compounds in a living organism. For this model spheroids from human endothelial cells (HUVECs) are implanted subcutaneously in mice in an extracellular matrix containing angiogenic growth factors. Subsequently, the formation of a human vasculature and the effect of treatment can be monitored in vivo and ex vivo. The in vivo angiogenesis model is suitable for testing the pro- or anti-angiogenic in vivo efficacy of antibodies or other biologicals and compounds.

Infos in Brief

Base Package

Implantation of spheroids from luciferase expressing HUVECs;
(i) administration of test compound (20 days);
(ii) bioluminescence imaging (1x / week);
(iii) measurement of ex vivo luciferase-activity at the end of the study;
(iv) protocol & report

Reference Cpd

Testing of Sunitinib included in studies with antiangiogenic agents

Optional Services

Implantation of spheroids from untransfected HUVECs;
(i) counting of human blood vessels by human CD34 staining;
(ii) determination of relative pericyte coverage and/or perfusion rate of human vessels

Variation of Test Model

Induction of vessel formation by pro-angiogenic factors or addition of cells

Summary Sheets

PDF Luciferase Analysis   PDF Classical Analysis