In Vivo Testing Services

Metastasis Tumor Models (Xenograft Models & Syngeneic Models)

Field of Application

Metastasis tumor models are designed to analyze the anti-metastasic effect of novel anti-cancer drug candidates. For this purpose luciferase-transduced tumor cells with high metastatic potential are either implanted orthotopically into their organs of origin, intravenously or intracardially. Metastasis formation can also be analyzed with bone marrow engraftment models, where luciferase expressing leukemia cell lines are implanted intravenously.

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Established Models

#

Tumor Cell Line

Tissue Origin

Route of Application

Species

Info Sheet

1

MOLM-13

Blood /Leukemia

i.v.

Human

2

MV4-11

Blood /Leukemia

i.v.

Human

3

4T1 (M3)*

Breast

orthotopic

Mouse

4

MDA-MB-231 (Z1)*

Breast

i.v.

Human

5

B16-F10

Skin

i.v.

Mouse

* in vivo selected subpopulation of the corresponding parental cell line

Infos in Brief

Animals Used

All studies are performed in mice

Analysis of Metastasis

Measurement of ex vivo luciferase activity in different organs

Reference Cpd

Available for most established models

Standard Study

Comprises among other things: (i) cell culturing & cell implantation; (ii) measurement of animal weight (3x / week); (iii) determination of tumor burdon via bioluminescence imaging (1x / week); (iv) treatment for 3 weeks (1x / day; 5x / week); (v) analysis of ex vivo luciferase activity in 4 organs upon necropsy; (vi) protocol & report

Optional Services

(i) Blood sampling; (ii) ex vivo analysis of additional organs; (iii) paraffin embedding of tumor/organ ; (iv) histology & pathology

Summary Sheet

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