Recombinant Proteins

Custom-Tailored Protein Production

Field of Application

If the kinase of your interest is not yet available as off-the-shelf product, we may produce this protein for you according to your specific requirements.

Our Service

Two Types of Custom-Tailored Protein Production

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Type of Production

Description of Work Package

1

small scale

Cloning, expression & purification of 100 μg of your protein of choice Quality control including Coomassie stain and determination of ATP-Km and Vmax (if applicable). In case no substrate is known, we perform a substrate finding approach testing at least 20 generic substrates.

2

large scale

Upscaling of expression and purification of mg amounts of your protein of choice Quality control including Coomassie stain, Mass Specs analysis, Activity measurement (ATP-Km, Vmax, IC50 of reference inhibitor; if available).

Infos in Brief

Expression Systems

Sf9 insect cells
E.coli

Available Tags

GST
HIS
GST-HIS
HALO-HIS
SUMO-HIS
TXN-HIS
Additional tags are available

Modification

Activation by autophosphorylation
Activation by upstream kinase
Removal of tag
Co-expression with co-factors
Mutations

Activity Assay Formats

FlashPlate™-based radiometric assay
Filter binding assay
ADP-Glo™ kinase assay, Promega