Cellular Assay Services

Cellular Phosphorylation Assays

Field of Application

Cellular activity of test compounds can differ from biochemically derived results. Therefore cellular testing should at least be part of rationale drug development. Our cellular phosphorylation assays have been designed to address compound activity in a physiological environment on a physiological substrate.

Our Service

Select Phosphorylation Assays

#

Kinase

Cell Line

Reference Compound

Info Sheet

1

AKT1 (h)

Rat1-myrAKT1 (r)

Staurosporine

2

ALK (h)

Karpas-299 (h)

Crizotinib

3

Aurora-B (h)

HT-29 (h)

Staurosporine

4

AXL (h)

MEF-AXL (m)

Sunitinib

5

BCR-ABL

K562

Dasatinib

6

B-RAF-VE (h)

Rat1-B-RAF-VE (r)

Sorafenib

7

EGF-R (h)

A431 (h)

Lapatinib

8

EGF-R G719S Mutant (h)

Rat1 (r)

Lapatinib

9

EGF-R L858R Mutant (h)

Rat1 (r)

Lapatinib

10

EGF-R L861Q Mutant (h)

Rat1 (r)

Lapatinib

11

EGF-R T790M Mutant (h)

Rat1 (r)

Lapatinib

12

EGF-R 790M/L858R Mutant (h)

Rat1 (r)

Lapatinib

13

EGF-R Δ752-759 Mutant (h)

Rat1 (r)

Lapatinib

14

EGF-R Δ747-749/A750P Mutant (h)

Rat1 (r)

Lapatinib

15

EGF-R control wt (h)

Rat1 (r)

Lapatinib

16

EPHB4 (h)

MEF-EPHB4 (m)

Sorafenib

17

ERBB2 (h)

NIH3T3-ERBB2 (m)

Lapatinib

18

ERBB4 (h)

T47D (h)

Lapatinib

19

FAK (h)

MEF-FAK (m)

Staurosporine

20

FGF-R2

Kato III

Vargatef

21

FLT3-wt (h)

MEF-FLT3-wt (m)

Sunitinib

22

FLT3-DY (h)

MEF-FLT3-DY (m)

Sunitinib

23

FLT3-ITD (h)

MEF-FLT3-ITD (m)

Sunitinib

24

FYN (h)

Rat1 (r)

Dasatinib

25

Haspin (GSG2)

HT-29 (h)

CHR-6494

26

IGF1-R (h)

MEF-IGF1-R (m)

Staurosporine

27

KIT (h)

M07e (h)

Sunitinib

28

MET (h)

MKN45 (h)

PHA665752

29

MET D1288H Mutant (h)

Rat1 (r)

BMS777607

30

MET D1228N Mutant (h)

Rat1 (r)

BMS777607

31

MET F1200l Mutant (h)

Rat1 (r)

BMS777607

32

MET M1250T Mutant (h)

Rat1 (r)

BMS777607

33

MET Y1230H Mutant (h)

Rat1 (r)

BMS777607

34

MET Y1230A Mutant (h)

Rat1 (r)

BMS777607

35

MET Y1230C Mutant (h)

Rat1 (r)

BMS777607

36

MET Y1230D Mutant (h)

Rat1 (r)

BMS777607

37

MET control wt (h)

Rat1 (r)

BMS777607

38

MNK1 (h)

Karpas-299 (h)

CGP-57380

39

PDGFR-beta (m)

NIH3T3 (m)

Sunitinib

40

PIM1

HEK293T (h)

AZD1208

41

PIM2

HEK293T (h)

AZD1208

42

PIM3

HEK293T (h)

AZD1208

43

ROCK (r)

A7r5 (r)

Y-27362

44

RON (h)

T47D (h)

Crizotinib

45

S6K (h)

Karpas-299 (h)

Everolimus

46

SRC (h)

MEF-SRC (m)

Dasatinib

47

TIE2 (h)

CHO-TIE2 (ha)

Sorafenib

48

VEGF-R2 (h)

HUE (h)

Sunitinib

49

VEGF-R3 (h)

MEF-VEGF-R3 (m)

Sunitinib

origin of kinase and cell line: (h) = human, (m) = mouse, (r) = rat, (ha) = hamster

Infos in Brief

Cpd Exposure

90 min

Readout

ELISA (sandwich or solid phase)

Testing Formats

IC50 (8 semi-log dilutions in duplicate)

Cpd Submission

Solved or as pre-weighed powder

Tolerable Solvent

Max 1 % DMSO

Report

Comprehensive evaluation of data including raw
data for own analysis

Summary Sheet

PDF