Cellular Assay Services

3D Tumor Spheroid Assays

Field of Application

This service can be used to analyze the effect of test compounds on three-dimensional cell clones either with mono-spheroids of only tumor cells or with co-spheroids of tumor and stroma cells. Since tumor and stroma cells are labelled with different luciferases the effect of test compounds on tumor and stroma cells can be analyzed separately.

Our Service

The following cell & test combinations are currently available for mono- or co-spheroid studies:

  Renilla Luciferase labelled stroma cells
none HS5 HS27 NHDF
Firefly
Luciferase
labelled
tumor
cells
none

A549

DLD1

HCT116

LN229

MCF7 0 0

U87MG

= good aggregates; 0 = loose association

Infos in Brief

Three Test Variants

(i) Mono-spheroids with only tumor cells; (ii) co-spheroids with tumor & stroma cells, luciferase measurement only in tumor cells; (iii) co-spheroids with luciferase labelled tumor & renilla labelled stroma cells

Number of Data Points for EC50 Measurement

8 concentration in duplicates in deca- or semi-logarithmic steps

-

Test Format

96-Well high-throughput format

Further Cell Lines

Can be established on request

Cpd Submission

Solved in 100% DMSO or as pre-weighted powder

Report

Comprehensive report including raw data for in-house analysis

Summary Sheet

PDF