ProQinase Tools &Tests: Services

iProKiTe^®-Package 3: Cellular Testing Services

Cellular Angiogenesis Assay

In a viscous medium endothelial cells (ECs) aggregate overnight to form a cellular spheroid. This behavior was used to establish a spheroid-based cellular angiogenesis assay. For this assay human EC spheroids are embedded in a defined matrix (e.g. collagen or fibrin), are stimulated and finally EC sprouting is quantified.

Pro-angiogenic compounds/growth factors induce EC sprouting; anti-angiogenic compounds (e.g. VEGF-A, FGF-2) inhibit growth factor induced EC sprouting.

The assay system is suitable for angiogenic/anti-angiogenic lead identification and optimization
ProQinase offers single concentration testing of anti-angiogenic compounds and standard IC50 determination of such compounds by testing at
7 concentrations in semi-log steps
Assays for potential angiogenic compounds can be designed according to customers' need

Cellular Phosphorylation Assays

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