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Cellular Phosphorylation Assays |
- The cellular phosphorylation assays have been developed in order to demonstrate kinase inhibition in an intact cellular background
- ProQinase screens your compound panel just at one concentration or performs IC50 determinations by testing your compounds at 8 concentrations in duplicate in semi-log steps
- The cellular phosphorylation assays are ideal to profile a set of compounds in parallel in different assays in order to select the best compound(s) for subsequent in vivo testing
Currently cellular testing services in cellular phosphorylation assays can be selected from the following assay systems
A PDF data sheet can be obtained for each cellular phosphorylation assay by clicking on the name of the kinase.
| Kinase Name |
Cell Line |
Transfected |
Type of Kinase |
Origin:
Kinase/Cell Line |
| AKT1 |
Rat1-myrAKT1 |
yes |
Ser/Thr Kinase |
human/rat |
| Aurora-B |
HT-29 |
no |
Ser/Thr Kinase |
human/human |
| AXL |
MEF-AXL |
yes |
RTK |
human/mouse |
| B-RAF-VE |
Rat1-B-RAF-VE |
yes |
Ser/Thr Kinase |
human/rat |
| EGF-R |
A431 |
no |
RTK |
human/human |
| EPHB4 |
MEF-EPHB4 |
yes |
RTK |
human/mouse |
| ERBB2 |
NIH3T3-ERBB2 |
yes |
RTK |
human/mouse |
| FAK |
MEF-FAK |
yes |
Non-receptor TK |
human/mouse |
| FLT3-wt |
MEF-FLT3-wt |
yes |
RTK |
human/mouse |
| FLT3-DY |
MEF-FLT3-DY |
yes |
RTK |
human/mouse |
| FLT3-ITD |
MEF-FLT3-ITD |
yes |
RTK |
human/mouse |
| IGF1-R |
MEF-IGF1-R |
yes |
RTK |
human/mouse |
| KIT |
M07e |
no |
RTK |
human/human |
| MET |
MKN45 |
no |
RTK |
human/human |
| PDGFR-beta |
NIH3T3 |
no |
RTK |
mouse/mouse |
| SRC |
MEF-SRC |
yes |
Non-receptor TK |
human/mouse |
| TIE2 |
CHO-TIE2 |
yes |
RTK |
human/hamster |
| VEGF-R2 |
HUE |
no |
RTK |
human/human |
Differential Cytotoxicity Assay
Spheroid-Based Cellular Angiogenesis Assay
Please inquire for a specific quote.
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cellular phosphorylation, cellular phosphorylation assay, cellular phosphorylation assays
Imprint | Copyright 2002-2010 ProQinase GmbH. All rights reserved. |
