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Cellular Phosphorylation Assays |
- The cellular phosphorylation assays have been developed in order to demonstrate kinase inhibition in an intact cellular background
- ProQinase screens your compound panel just at one concentration or performs IC50 determinations by testing your compounds at 8 concentrations in duplicate in semi-log steps
- The cellular phosphorylation assays are ideal to profile a set of compounds in parallel in different assays in order to select the best compound(s) for subsequent in vivo testing
Prices for Cellular Phosphorylation Assay Services
Description |
Price (EUR) |
Price (USD) |
Project Base Fee
(for setup, reference IC50 and report, for standard projects with up to 4 kinases) |
400 |
520
|
Price per IC50
(8 concentrations in duplicate; semi-log steps) |
250 |
330 |
Price per Single Concentration
(1 test compound in duplicate, for standard projetcs with at least 24 test compounds) |
35 |
45
|
Example 1 : IC50 determination of 12 test compounds against 3 different kinases (36 IC50 curves)
400 EUR + (12 x 3 x 250 EUR) = 9,400 EUR
Example 2: Single concentration measurement of 480 test compounds against 1 kinase
400 EUR + (480 x 35 EUR) = 17,200 EUR
Please inquire for a specific quote.
| Kinase Name |
Cell Line |
Trans-fected |
Data
Sheet |
Type of Kinase |
Origin:
Kinase/Cell Line |
| AKT1 |
Rat1-myrAKT1 |
yes |
|
Ser/Thr Kinase |
human/rat |
| Aurora-B |
HT-29 |
no |
|
Ser/Thr Kinase |
human/human |
| AXL |
MEF-AXL |
yes |
|
RTK |
human/mouse |
| B-RAF-VE |
Rat1-B-RAF-VE |
yes |
|
Ser/Thr Kinase |
human/rat |
| EGF-R |
A431 |
no |
|
RTK |
human/human |
| EPHB4 |
MEF-EPHB4 |
yes |
|
RTK |
human/mouse |
| ERBB2 |
NIH3T3-ERBB2 |
yes |
|
RTK |
human/mouse |
| FAK |
MEF-FAK |
yes |
|
Non-receptor TK |
human/mouse |
| FLT3-wt |
MEF-FLT3-wt |
yes |
|
RTK |
human/mouse |
| FLT3-DY |
MEF-FLT3-DY |
yes |
|
RTK |
human/mouse |
| FLT3-ITD |
MEF-FLT3-ITD |
yes |
|
RTK |
human/mouse |
| IGF1-R |
MEF-IGF1-R |
yes |
|
RTK |
human/mouse |
| KIT |
M07e |
no |
|
RTK |
human/human |
| MET |
MKN45 |
no |
|
RTK |
human/human |
| PDGFR-beta |
NIH3T3 |
no |
|
RTK |
mouse/mouse |
| SRC |
MEF-SRC |
yes |
|
Non-receptor TK |
human/mouse |
| TIE2 |
CHO-TIE2 |
yes |
|
RTK |
human/hamster |
| VEGF-R2 |
HUE |
no |
|
RTK |
human/human |
Differential Cytotoxicity Assay
Spheroid-Based Cellular Angiogenesis Assay
back |
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