ProQinase's Drug Discovery Products & Services are uniquely designed for drug development targeting protein kinases in cancer and other therapeutic areas. In oncology one particular focus is the performance of angiogenesis assays. Presently the following products are available and the following biochemical, cellular and in vivo services are offered.

ProQinase offers in-house produced recombinant protein kinases for activity assays. Currently 239 active protein kinases including more than 60 mutant kinases are available as off-the-shelf products. In addition, additional protein kinase variants (inactive or non-activated) are available from ProQinase. Kinases can also be produced as custom-tailored proteins according to your requirements. Moreover, ProQinase offers recombinant substrates for biochemical kinase activity assays.

 Biochemical Kinase Assay Services

ProQinase offers different types of compound testing in biochemical assays with protein kinases and lipid kinases. Our highly flexible free choice kinase assay projects include high throughput screening (HTS) of large compound libraries against one target, and selectivity profiling against several or all kinases which are available at ProQinase. Testing can be performed either as single concentration measurements or as IC50 determinations.
In addition to  free choice kinase assay projects the Kinase WholePanelProfiler (358 protein kinases) and the Kinase WholePanelProfiler^Plus (358 protein kinases and 7 lipid kinases) allow to profile test compounds against broad panels of kinases.
Moreover, ProQinase offers to test your compound(s) in predefined Kinase MutantProfiler comprising different mutants and the wildtyp form of a given kinase and including a side-by-side comparison with a reference inhibitors.
The KinaseFinder is offered as a standardized service package which enables to identify protein kinases that phosphorylate a protein or peptide of interest.
The Kinase SubstrateFinder is another standardized service package that allows identification of protein kinase substrates for biochemical protein kinase activity assays.

ProQinase offers several types of cellular assay services to characterize compounds on the cellular level. On the one hand, we offer cellular phosphorylation assays  to assess the inhibitory effect of compounds on a specific kinase (currently established for 22 different kinases). On the other hand, we offer various sophisticated phenotypic assays, such as (i) the spheroid-based angiogenesis assay to evaluate the angiogenic or anti-angiogenic potential of compounds, (ii) the cell migration assay which allows to identify the impact of compounds on the motility of various cell lines, and (iii) the soft agar assay (or clonogenic assay) which characterizes compounds for their ability to inhibit cell transformation.
In all types of assays testing can be performed in screening mode (using only one or a few concentrations of the test compounds) or as IC50 determination.

ProQinase has established a large panel of subcuteanous xenografts and orthotopic in vivo tumor models. Most orthotopic tumor studies are performed with luciferase transfected tumor cell lines allowing bioluminescene imaging. ProQinase's unique spheroid-based in vivo angiogenesis assay allows determination of the anti-angiogenic effect of compounds or antibodies against functional human blood vessels in mice.

Individual service packages fitting your individual needs are available on request.