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Subcutaneous Tumor Models (Xenografts) |
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The test models
Subcutaneously implanted tumor cells represent a convenient means to test novel potential anticancer drugs in vivo. A large variety of human and murine cell lines derived from both, solid tumors or leukemias, covering a wide range of tumor geno- and phenotypes, have been adapted to grow in a murine host, and thus allow testing of a compound in the appropriate tumor model.
Tumor cells harvested from tissue culture flasks are implanted into the subcutaneous space of the left flank of the mice. Resulting subcutaneous tumors will be monitored by calipering. Animal weights will be measured three times weekly. Animal behaviour will be monitored daily. All tumor tissues will be preserved for determination of tumor weight and tumor volumes at necropsy. The mice are maintained in separated isolated housing at constant temperature and humidity.
More cell lines which are availabel from ATCC or other sources, can easily be established if they are tumorigenic in mice. |
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Tissue-Derived Models
| Tissue Origin |
Tumor Cell Line |
Species |
Fact
Sheet |
| Blood/Leukemia |
HL-60 |
Human |
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KARPAS-299 |
Human |
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MOLM-13 |
Human |
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MV4-11 |
Human |
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| Bone |
SJSA-1 |
Human |
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| Brain |
C6 |
Rat |
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SK-N-MC |
Human |
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| Breast |
MCF7 |
Human |
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MDA-MB-231 |
Human |
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| Cervix |
C33A |
Human |
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HeLa |
Human |
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| Colon |
Colo-205 |
Human |
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HCT-116 |
Human |
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HT29 |
Human |
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LoVo |
Human |
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LS 174T |
Human |
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RKO |
Human |
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SW620 |
Human |
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CT26.wt |
Mouse |
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| Gastric |
MKN-1 |
Human |
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MKN-45 |
Human |
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| Kidney |
RENCA |
Mouse |
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| Liver |
Alexander |
Human |
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| Lung |
H460 |
Human |
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A549 |
Human |
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NCI-H69 |
Human |
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Calu-6 |
Human |
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| Ovary |
OVCAR-3 |
Human |
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SKOV-3 |
Human |
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| Pancreas |
AsPC1 |
Human |
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MiA-PaCa2 |
Human |
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| Prostate |
LNCaP (Z2)* |
Human |
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PC-3 |
Human |
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| Skin |
A375 |
Human |
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A431 |
Human |
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B16-F10 |
Mouse |
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*): in vivo selected subpopulation of LNCaP
Target-Specific Tumor Models with Genetically Engineered Cells
| Target |
Tumor Cell Line |
Species |
Fact
Sheet |
| IGF1 Receptor |
MEF-IGF1-Rrep* |
Mouse |
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| ErbB2 Receptor |
NIH3T3-ErbB2-Rrep* |
Mouse |
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*): Contains technology which is protected by US Patent 5,464,758 owned by TET Systems GmbH & Co. KG, Germany
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Study example
In the example shown here, one group of mice bearing subcutaneous MV4-11 xenografts was treated with VX680, the other group with vehicle only. Treatment started after randomisation when tumor volumes had reached a size of approximately 100-200 mm3.
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In vivo efficacy study using VX-680 and vehicle
control in a subcutaneous MV4-11 xenograft model
[click here to enlarge image]
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Our service
Base package
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Culturing of the required cell line
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Subcutaneous implantation of tumor cells
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Measurements of animal weight, tumor weight (at necropsy), tumor volume
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Administration of test compound (e.g. p.o., i.p., s.c., i.v.) for approx. 21 days
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Blood sampling
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Shipment of harvested tumor tissues
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Preparation of detailed report
Optional
- Pretesting of target expression on tumor sections of paraffin-embedded or frozen tissue
- Analysis of “Tissue Microarrays” with tumor tissues of the different available in vivo models
- Fixation of tissues using different fixation agents
- Paraffin embedding of collected tissues using an automated system (Leica TP 1020 and Leica EG 1160)
- Histological sectioning of paraffin-embedded (Leica SM 2000R) or cryoconserved tissues (Leica CM 3050)
- Hematoxylin-Eosin staining of tissue slides for histological analysis
- Selected immunohistochemistry analysis
- Custom-tailored composition of tumor tissues of an entire in vivo study to a single “Tissue Microarray” for further analysis.
Please inquire to receive a custom-tailored quote according to your individual requirements.
Summary fact sheet
ProQinase's service portfolio of subcutaneous tumor models (xenografts)
Related Products and Services:
Orthotopic Tumor Models
In vivo Angiogenesis Assay
Tissue Microarray (TMA)
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